e coli k12 mg1655 (ATCC)
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e coli k12 mg1655
E Coli K12 Mg1655, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1578 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e+coli+k12+mg1655/pmc12811044-61-35-45?v=ATCC
Average 99 stars, based on 1578 article reviews
E Coli K12 Mg1655, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1578 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e+coli+k12+mg1655/pmc12811044-61-35-45?v=ATCC
Average 99 stars, based on 1578 article reviews
e coli k12 mg1655 - by Bioz Stars,
2026-08
99/100 stars
Images
1) Product Images from "Hurdles to horizontal gene transfer: species-specific effects of synonymous variation and plasmid copy number determine antibiotic resistance phenotype"
Article Title: Hurdles to horizontal gene transfer: species-specific effects of synonymous variation and plasmid copy number determine antibiotic resistance phenotype
Journal: Microbiology
doi: 10.1099/mic.0.001652
Figure Legend Snippet: aacC1 synonymous variant design and constructs. ( a ) First 34 codons of the synonymous variant sequences. The first 10 codons (30 bp) were conserved across variants. The remaining codons were assigned different synonymous codons in a semi-random manner, guided by the codon usage of the bacterial species used. Pairwise identity with wt aacC1 is shown to the right of each sequence. ( b ) Variant constructs were composed of an integron-derived promoter and 5′ UTR, followed by the aacC1 gene and cloned into a pBBR1 broad-host-range plasmid. Variant plasmid sequences differed only by the variable region within aacC1 . ( c ) Codon usage similarity (COUSIN) for the 32 synonymous aacC1 variants in the three host species used. Variants are ordered by increasing COUSIN values in E. coli . GC content at the third base of each codon (GC3) is represented by bubble size. The five variants that could not be transformed into A. baylyi are circled in red on the x-axis. Equivalent figures ordered by increasing COUSIN values in A. baylyi and P. aeruginosa are provided as Fig. S2B and C.
Techniques Used: Variant Assay, Construct, Sequencing, Derivative Assay, Clone Assay, Plasmid Preparation, Transformation Assay
Figure Legend Snippet: Synonymous variants confer different resistance levels. ( a ) Resistance levels are represented by IC50 AUC , i.e. the concentration of gentamicin required to reduce the AUC by 50% compared to growth in the absence of gentamicin. Synonymous variants are ordered on the x-axis by increasing IC50 AUC in E. coli . ( b ) PCN, quantified by qPCR, in each host variant. Significant differences in PCN are indicated by ***, and non-significant differences are indicated by ns. ( c ) AUC of the three bacteria species carrying the aacC1 variants in 50 µg ml −1 and 400 µg ml −1 of gentamicin. AUC is represented as relative to AUC in 0 µg ml −1 gentamicin. Some variant*species combinations were not tested at each of the concentrations. The AUC of these combinations was set to 0 when the variant*combination did not grow at a lower gentamicin concentration and to 1 when the variant*combination had, in the presence of a higher concentration of gentamicin, an AUC equivalent to the one in the absence of gentamicin. White boxes indicate the five variants that could not be transformed in A. baylyi .
Techniques Used: Concentration Assay, Variant Assay, Bacteria, Transformation Assay
Figure Legend Snippet: CUPs and resistance. Pearson correlation between resistance (IC50 AUC ) and tAI (first line), COUSIN (second line), CAI (third line) and |Δ(GC3)| (fourth line) for A. baylyi (first column), E. coli (second column) and P. aeruginosa (third column). Significant correlations ( P <0.05) are represented by a black regression line. Non-significant Pearson correlations are represented by a grey dotted regression line.
Techniques Used:
